Isolation and identification of urinary tract Infectious proteus mirabilis

Pr. mirabilis causes several infections in patients with 'complicated' urinary tract infections. In cases where stones are formed, the bacteria can become resistant to antibiotics. Pr. mirabilis can be diagnosed in the laboratory due to its characteristic swarming motility, and inability to metabolize lactose on a MacConkey agar plate. The identification and antibiotic sensitivity of hundred and two urine samples [38 males, 64 females] with urinary tract infection [UTIs] were investigated. Sixty samples, [59%] had associated urinary tract infection with Pr. mirabilis which has the most common causative microorganism. The bacterial isolates altered in their susceptibility to antimicrobial agents. Indole test, methyl red test, trytophan test, oxidase test, and casein test were appeared to be negative but the catalase test, phenylalanine deaminase test, urea test, hydrogen suflide test and citrate agar test were exerted a positive results

Microbiological studies on beta-lactamase activity in staphylococcus aureus

The study of susceptibility of 92 Gram - positive bacteria to some antibiotics revealed that 27.2%, 96.0%, 13%, 100%, 7.6%, 31.5% and 17.4% of the total 92 isolates were resistant to methicillin, penicillin, tetracycillin, amoxicillin, chloramphenicol, cephradin and streptomycin respectively and only six isolates had high level of MIC ranging from 1500 to 7000 micro g/ml amoxicillin and had ability to produce beta -lactamase activity but they differ in their activities. Therefore cultural, morphological and physiological characteristic of these isolates were studied and these results indicated that they belong to Staphylococcus aureus. One isolate showed the highest level of resistance [MIC] and this isolate produced highest beta -lactamase activity. beta -lactamase activity can be affected by subminimal inhibitory concentration of some antibiotics, the maximal inhibitory effect was induced by concentration ranging from 1/2 to 1/32 of the minimal inhibitory concentration of streptomycin, clindamycin and chloramphenicol